Histochemistry and scanning electron microscopy of tooth integumental material in humans

Plaque material on human teeth was investigated using histochemical techniques and scanning electron microscopy. A positive reaction for protein, cargohydrate, and lipids was seen around the fissures and in a well-defined band around the sides of the tooth corresponding to the highest contour. These regions are the location of dense aggregates of bacteria as seen with scanning electron microscopy. This study emphasizes the fact that considerable attention should be given to the cleaning of not only the occlusal surfaces but also the highest contour of the tooth.