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Related Experiment Videos

Acetylcholine responses in L cells.

P G Nelson, J H Peacock

    Science (New York, N.Y.)
    |September 15, 1972
    PubMed
    Summary
    This summary is machine-generated.

    Mouse L cells exhibit a unique hyperpolarizing activation response to mechanical or electrical stimuli. This response, also triggered by acetylcholine, suggests neurotransmitter sensitivity and non-cholinergic intercellular communication in these cell lines.

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    Area of Science:

    • Cell biology
    • Neuroscience
    • Electrophysiology

    Background:

    • L cells are continuous cell lines of mouse fibroblastic origin.
    • These cells exhibit a prolonged active membrane hyperpolarization upon stimulation.

    Purpose of the Study:

    • To investigate the nature of the hyperpolarizing activation response in L cells.
    • To determine the mechanisms of intercellular communication and neurotransmitter sensitivity.

    Main Methods:

    • Mechanical and electrical stimulation of L cells.
    • Iontophoretic application of acetylcholine.
    • Pharmacological blockade using atropine, curare, and modified bathing solutions.

    Main Results:

    • L cells generate a prolonged active membrane hyperpolarization (hyperpolarizing activation response) when mechanically or electrically stimulated.

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  • Acetylcholine elicits a similar response, which is blocked by atropine.
  • The response propagates between adjacent cells independently of direct electrical coupling and is not blocked by atropine or curare.

    • Conclusions:

    • L cells are capable of generating an active electrical response.
    • L cells demonstrate sensitivity to acetylcholine, a neurotransmitter.
    • Humoral, likely non-cholinergic, interaction occurs between L cells.