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Cell surface immunoglobulin. V. Release from murine splenic lymphocytes
The Journal of Experimental Medicine
|October 1, 1972
Summary
Small lymphocytes secrete immunoglobulin M (IgM) and immunoglobulin G (IgG). Cell surface IgM is released into the medium, while IgG primarily exists intracellularly, informing a novel Ig transport model.
Area of Science:
- Immunology
- Cell Biology
Background:
- Small lymphocytes play a crucial role in immune responses.
- Understanding immunoglobulin (Ig) transport and secretion is vital for immunology.
Purpose of the Study:
- To investigate the turnover and release mechanisms of cell surface Ig.
- To study the secretion of intracellular Ig in small lymphocytes.
- To propose a model for Ig transport and release.
Main Methods:
- Incubation of normal mouse spleen lymphocytes.
- Analysis of secreted and cell surface Ig.
- Temperature-dependent release studies.
- Biochemical characterization of released Ig.
Main Results:
- Small lymphocytes secrete 8S IgM and IgG.
- Cell surface IgM undergoes active turnover (half-life 6-8 hr) via temperature-dependent release.
- Released cell surface Ig is associated with plasma membrane fragments.
- H-2 antigens are not released during short-term incubation.
Conclusions:
- A model for the transport and release of both cell surface and secreted Ig is proposed.
- Lymphocyte Ig secretion involves distinct pathways for IgM and IgG.
- Cell surface IgM turnover is a dynamic, regulated process.
