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Related Experiment Videos

Electron microscope observations on myosin from Physarum polycephalum.

V T Nachmias

    The Journal of Cell Biology
    |March 1, 1972
    PubMed
    Summary

    Researchers isolated myosin from Physarum polycephalum actomyosin, confirming previous findings. This study details myosin

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Molecular Biology

    Background:

    • Physarum polycephalum is a model organism for studying muscle contraction.
    • Myosin and actin interactions are fundamental to cellular motility.

    Purpose of the Study:

    • To isolate and characterize myosin from Physarum polycephalum.
    • To investigate the aggregation properties of Physarum myosin and its interaction with actin.

    Main Methods:

    • Isolation of myosin from Physarum polycephalum actomyosin.
    • Electron microscopy for examining negatively stained preparations.
    • Biochemical assays for calcium ATPase activity.

    Main Results:

    • Physarum myosin was successfully isolated with significant calcium ATPase activity.
    • Physarum myosin formed polarized arrowhead complexes with rabbit skeletal muscle actin.
    • Myosin alone did not form filaments, but myosin-enriched actomyosin showed aggregation in the presence of ATP.

    Conclusions:

    • Actin-myosin interactions in Physarum favor head-to-tail aggregation.
    • The tail portions of Physarum myosin may play a role in filament formation.
    • Physarum myosin exhibits unique aggregation properties compared to muscle myosin.

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