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Transformation in group H streptococci: blackburn strain transformable mutants
Journal of Bacteriology
|May 1, 1972
Summary
Scientists isolated transformable mutants of group H streptococci. These mutants, unlike the wild strain, possess cell surface factors that inactivate deoxyribonucleic acid (DNA) in laboratory tests.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- Group H streptococci are typically nontransformable.
- Understanding genetic exchange mechanisms in bacteria is crucial.
Purpose of the Study:
- To isolate and characterize transformable mutants of group H streptococci.
- To investigate the properties of these mutants compared to the wild strain.
Main Methods:
- Isolation of bacterial mutants.
- Cell surface extraction.
- In vitro deoxyribonucleic acid inactivation assays.
Main Results:
- Transformable mutants of the Blackburn strain group H streptococci were successfully isolated.
- Cell surface extracts from these mutants, unlike the wild strain, contained a factor that inactivated deoxyribonucleic acid in vitro.
Conclusions:
- The isolated mutants possess unique cell surface characteristics.
- A deoxyribonucleic acid-inactivating factor present on the mutant cell surface may play a role in their transformability or affect genetic material.