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Related Experiment Videos

An active electrical response in fibroblasts.

P G Nelson, J Peacock, J Minna

    The Journal of General Physiology
    |July 1, 1972
    PubMed
    Summary
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    Mechanical and electrical stimuli trigger a hyperpolarizing activation (H.A.) response in mouse L cells, involving a significant increase in membrane permeability, primarily to potassium ions.

    Area of Science:

    • Cell biology
    • Electrophysiology

    Background:

    • Mouse L cells exhibit a resting membrane potential of -16 mV at 37°C.
    • Membrane resistivity is approximately 20,000 ohmcm² when considering surface filopodia.

    Purpose of the Study:

    • To characterize the hyperpolarizing activation (H.A.) response in mouse L cells, 3T3 cells, and normal fibroblasts.
    • To investigate the ionic mechanisms underlying the H.A. response.

    Main Methods:

    • Electrophysiological recordings of membrane potential and permeability changes.
    • Stimulation using mechanical and electrical methods.
    • Manipulation of external potassium ion concentration.

    Main Results:

    • Mechanical and electrical stimuli induced a prolonged (3-5 sec) H.A. response, increasing membrane permeability (2-10 fold) and hyperpolarizing the membrane to -50 mV.

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  • The reversal potential for the H.A. response shifted from -80 mV to -40 mV when external potassium increased from 5.4 mM to 27 mM.
  • The H.A. response involves a significant increase in membrane permeability, predominantly to potassium ions.

    • Conclusions:

    • The H.A. response represents a substantial increase in membrane permeability, primarily to potassium.
    • The observed changes in reversal potential confirm the dominant role of potassium ions in the H.A. response.