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Related Experiment Videos

Ribonuclease activities in developing experimental granulation tissue with reference to polysomes.

S Aho, E Kulonen

    Acta Physiologica Scandinavica
    |July 1, 1979
    PubMed
    Summary

    Polysomes in granulation tissue peak at three weeks, with younger tissue showing higher protein synthesis activity. High ribonuclease (RNase) activity complicates polysome analysis in this tissue.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Wound Healing Research

    Background:

    • Granulation tissue formation is crucial for wound repair.
    • Polysomes are key to protein synthesis during tissue regeneration.
    • Understanding polysome dynamics and associated enzyme activity is vital for regenerative medicine.

    Purpose of the Study:

    • To investigate the changes in Mg2+-precipitable polysomes during experimental granulation tissue development.
    • To compare the protein synthesis activity of polysomes from young versus old granulation tissue.
    • To characterize the ribonuclease (RNase) activity associated with granulation tissue polysomes and supernatants.

    Main Methods:

    • Isolation and quantification of Mg2+-precipitable polysomes from granulation tissue at different time points.

    Related Experiment Videos

  • Assessment of cell-free protein synthesis activity of isolated polysomes.
  • Assay of ribonuclease (RNase) activity in polysomes and post-polysomal supernatants.
  • Investigation of the effect of p-chloromercuribenzoate (pCMB) on RNase activity.

    • Main Results:

    • Mg2+-precipitable polysomes increase up to three weeks and then decline.
    • Polysomes from younger granulation tissue exhibit higher protein synthesis efficiency.
    • Highest RNase activity is found in polysomes from 1-week tissue, containing both acid and latent alkaline RNase (activated by pCMB).
    • Post-polysomal supernatant shows peak RNase activity in 2-4 week old tissue, optimal at pH 7-8 and minimally affected by pCMB.

    Conclusions:

    • Granulation tissue exhibits dynamic changes in polysome content and activity during healing.
    • Elevated RNase activity, particularly in polysomes, poses challenges for analyzing polysome function.
    • Characterization of RNase forms and their modulation by factors like pCMB is important for understanding protein synthesis regulation in wound repair.