Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rates of plasma protein synthesis by deconvolution.

K H Norwich

    The Biochemical Journal
    |March 1, 1972
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a deconvolution method to accurately measure plasma protein synthesis rates. By correcting appearance curves with disappearance data, researchers can isolate synthesis from destruction and distribution.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Context effects in the entropic theory of perception.

    The Behavioral and brain sciences·2014
    Same author

    Determination of saltiness from the laws of thermodynamics--estimating the gas constant from psychophysical experiments.

    Chemical senses·2001
    Same author

    The tissue distribution of tumor necrosis factor-alpha in rats: a compartmental model.

    Metabolism: clinical and experimental·2000
    Same author

    Kinetics of T cell turnover following thymectomy.

    Cell proliferation·1999
    Same author

    Unification of psychophysical phenomena: the complete form of Fechner's law.

    Perception & psychophysics·1997
    Same author

    Noncompartmental models of whole-body clearance of tracers: a review.

    Annals of biomedical engineering·1997

    Area of Science:

    • Biochemistry
    • Physiology
    • Metabolic Research

    Background:

    • Measuring plasma protein synthesis is crucial for understanding metabolic processes.
    • Existing methods often struggle to differentiate synthesis from degradation and distribution.

    Purpose of the Study:

    • To develop a corrected method for measuring plasma protein synthesis rates.
    • To accurately quantify protein synthesis by accounting for catabolism and distribution.

    Main Methods:

    • Utilized (14)C-labelled arginine guanidine protein precursor (Na(2) (14)CO(3)) for tracing.
    • Measured plasma specific radioactivity over time to generate 'appearance' and 'disappearance' curves.
    • Applied deconvolution techniques to correct the 'appearance' curve using the 'disappearance' curve data.

    Related Experiment Videos

    Main Results:

    • The 'appearance' curve reflects a balance of protein synthesis, delivery, destruction, and distribution.
    • The 'disappearance' curve quantifies the decline in radioactivity from exogenously labeled protein.
    • The deconvolution method successfully corrects the appearance curve to isolate the synthesis rate.

    Conclusions:

    • The developed deconvolution method provides a more accurate measure of plasma protein synthesis.
    • This technique allows for precise quantification of protein synthesis independent of degradation and distribution.
    • Accurate synthesis rate measurement is vital for metabolic and physiological research.