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Purification and characterization of agrocin 84
Antimicrobial Agents and Chemotherapy
|September 1, 1979
Summary
A new purification method for agrocin 84, a compound from Agrobacterium radiobacter, yields 65% pure product. This rapid procedure utilizes multiple chromatographic techniques for efficient isolation.
Area of Science:
- Microbiology
- Biochemistry
Background:
- Agrocin 84 is a bacteriocin-like compound produced by Agrobacterium radiobacter strain K-84.
- Efficient purification methods are crucial for studying such compounds.
Purpose of the Study:
- To develop a rapid procedure for purifying milligram quantities of agrocin 84.
- To characterize the purified agrocin 84.
Main Methods:
- Charcoal adsorption
- Ion-exchange chromatography
- Sieving chromatography
- Continuous-flow electrophoresis
Main Results:
- A procedure yielding agrocin 84 with 65% purity on a dry weight basis was developed.
- Purified agrocin 84 exhibited strong UV absorption at 264 nm.
- Analysis revealed an unusual phosphoramidate linkage, with adenine, glucose, and phosphate in a 1:1:2 molar ratio.
- Estimated molecular weight of agrocin 84 is 1,350.
Conclusions:
- The developed procedure offers a rapid and effective means for agrocin 84 purification.
- The characterization data provide insights into the unique chemical structure of agrocin 84.