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Bacteriocin production by transformable group H streptococci.

R Schlegel, H D Slade

    Journal of Bacteriology
    |November 1, 1972
    PubMed
    Summary
    This summary is machine-generated.

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    Group H streptococci release a bacteriocin, streptocin (STH(1)), that kills other streptococci. This molecule, likely a protein-lipid complex, may necessitate reevaluating prior transformation studies.

    Area of Science:

    • Microbiology
    • Bacteriology
    • Molecular Biology

    Background:

    • Group H streptococci exhibit natural competence for genetic transformation.
    • Strain Challis, when competent, produces a substance lethal to other Group H streptococci.
    • Previous studies may not have accounted for this bacteriocin's activity.

    Purpose of the Study:

    • To characterize the bacteriocin produced by competent Group H streptococci (strain Challis).
    • To determine the nature and potential role of this bacteriocin in bacterial interactions.
    • To assess the implications of this bacteriocin for existing transformation data.

    Main Methods:

    • Bacteriocin production by strain Challis in liquid culture.
    • Enzyme sensitivity assays (deoxyribonuclease, ribonuclease, trypsin, phospholipase C, alkaline phosphatase) to probe molecular composition.

    Related Experiment Videos

  • Distinguishing bacteriocin activity from competence factor and bacteriophage.
  • Testing bacteriocin's effect on the competence of strain Wicky.

    • Main Results:

    • A bacteriocin, designated streptocin (STH(1)), was isolated from competent strain Challis.
    • STH(1) demonstrated resistance to nucleases but sensitivity to trypsin, phospholipase C, and alkaline phosphatase.
    • Enzyme sensitivity suggests STH(1) is a complex molecule containing protein, lipid, and essential phosphate groups.
    • STH(1) activity was distinct from competence factor and bacteriophage and did not induce competence in strain Wicky.

    Conclusions:

    • The bacteriocin STH(1) produced by competent Group H streptococci is a complex molecule.
    • Its properties suggest a protein-lipid structure with critical phosphate groups.
    • The existence of STH(1) necessitates a reevaluation of previous research on the Challis-Wicky transformation system.