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Bacteriological and pathological study of animals given Freund adjuvant.
Applied Microbiology
|October 1, 1972
Summary
Freund complete adjuvant containing Mycobacterium butyricum caused granulomatous lesions in rabbits and mice. Acid-fast bacilli were found in lesions, and M. butyricum was re-isolated, confirming its role in the observed pathology.
Area of Science:
- Immunology
- Microbiology
- Pathology
Background:
- Freund complete adjuvant (FCA) is widely used in immunological research.
- The role of Mycobacterium butyricum in FCA-induced pathology requires clarification.
Purpose of the Study:
- To investigate the pathological effects of FCA containing Mycobacterium butyricum.
- To confirm the presence and viability of Mycobacterium butyricum in induced lesions.
Main Methods:
- Intramuscular and intraperitoneal inoculation of rabbits and mice with FCA.
- Histopathological examination of lesions.
- Bacteriological culture and re-isolation of Mycobacterium butyricum.
Main Results:
- FCA induced local granulomatous lesions and visceral lesions in experimental animals.
- Acid-fast bacilli were identified within these lesions.
- Mycobacterium butyricum was successfully cultured from lesions and caused similar pathology upon re-inoculation.
Conclusions:
- Mycobacterium butyricum in FCA is directly responsible for the observed granulomatous pathology.
- The study confirms the infectious nature of the lesions induced by FCA.