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Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
Published on: March 23, 2018
Plate-slide for the culture and morphological observation of fungi
Abstract:
Adaptation of a polystyrene plate-slide is extremely well suited for the simultaneous macroscopic and microscopic diagnosis of fungi. This technique obviates the disorientation of identifying structures when cultures are mechanically disaggregated during preparation of slides for microscopy.
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Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
Overview of Fungi