Glycoproteins from experimental granulation tissue and their effects on collagen synthesis in embryonic chick tendon...

Buffer-soluble and pronase-liberated glycoproteins from experimental granulation tissue were fractionated by gel filtration and DEAE-cellulose chromatography. The age of the granuloma was reflected in the gel filtration pattern. Two glycoproteins were isolated, purified to homogeneity and analyzed for their carbohydrate and amino acid compositions. The collagen synthesis in embryonic chick tendon cells was measured in the presence of these fractions, which were found to be inhibiting even at 10(-6) M. These glycoproteins may be significant in the feedback regulation of the development of granulation tissue and fibrosis.