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Enzyme Kinetics

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Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
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Related Experiment Video

Updated: Jul 10, 2026

A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9

A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9

Published on: August 28, 2018

Lysozyme turnover in the rat.

N E Hansen, H Karle, V Andersen

The Journal of Clinical Investigation

|July 1, 1971

Summary

This summary is machine-generated.

Plasma lysozyme in rats is rapidly cleared, with neutrophils being a primary source. Kidneys are the main site for lysozyme storage and breakdown, releasing degradation products back into circulation.

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Last Updated: Jul 10, 2026

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Measuring Diurnal Rhythms in Autophagic and Proteasomal Flux

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Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

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Published on: April 6, 2022

Area of Science:

Biochemistry
Physiology
Immunology

Background:

Lysozyme is a key enzyme in innate immunity.
Understanding lysozyme turnover is crucial for comprehending immune cell homeostasis.

Purpose of the Study:

To investigate the turnover rate and organ distribution of lysozyme in rats.
To identify the primary source and primary site of degradation for plasma lysozyme.

Main Methods:

Utilized (125)I-labeled rat lysozyme to track its distribution and clearance.
Measured enzymatic lysozyme activity in various organs and biological fluids.
Calculated the rate of lysozyme synthesis.

Main Results:

Plasma lysozyme exhibited a rapid disappearance rate with a half-life of 75 minutes.
Neutrophilic granulocytes were identified as the likely main source of plasma lysozyme.
The kidneys were identified as the predominant organ for lysozyme storage and destruction.
Lysozyme slowly disappeared from kidneys over 4 days, with potential release of degradation products.

Conclusions:

The study elucidates the dynamic turnover of lysozyme in rats.
Neutrophil degradation is a significant contributor to plasma lysozyme levels.
The kidney plays a critical role in lysozyme catabolism and clearance.