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Cellular co-operation by mouse lymphocytes to chemically defined antigens
When populations of mouse spleen cells were treated with a highly radioactive 125I-labelled haptenic conjugate (125I-labelled PDG-N) and subsequently injected into X-irradiated, immunized (N-10-C) syngeneic BALB/c mice, it was found that after autoradiography with 125I-labelled N and C haptenic conjugates, the numbers of ABC to both the N and C haptens decreased. The number of ABC found were significantly lower than those found in mice that had been reconstituted with cells "suicided" with another 125I-labelled antigen (125I-labelled BSA) and subsequently immunized with N-10-C.
When populations of mouse spleen cells were treated with a highly radioactive 125I-labelled haptenic conjugate (125I-labelled PDG-N) and subsequently injected into X-irradiated, immunized (N-10-C) syngeneic BALB/c mice, it was found that after autoradiography with 125I-labelled N and C haptenic conjugates, the numbers of ABC to both the N and C haptens decreased. The number of ABC found were significantly lower than those found in mice that had been reconstituted with cells "suicided" with another 125I-labelled antigen (125I-labelled BSA) and subsequently immunized with N-10-C.