Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tissue PO2 during reanimation with hemoglobin solutions.

K Messmer, F Jesch, B Endrich

    European Surgical Research. Europaische Chirurgische Forschung. Recherches Chirurgicales Europeennes
    |January 1, 1979
    PubMed
    Summary

    Pyridoxylated stoma-free hemoglobin (SFH-PLP) improved tissue oxygenation in dogs after severe blood loss, showing promise as a short-term blood substitute. Conventional stoma-free hemoglobin (SFH) did not demonstrate the same oxygen-unloading benefits.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [DRG exercises: blunt abdominal trauma].

    Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2008
    Same author

    [DRG practice: wound management with vacuum therapy].

    Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2008
    Same author

    Peri-operative anaesthetic management of cytoreductive surgery with hyperthermic intraperitoneal chemotherapy.

    Anaesthesia·2008
    Same author

    [DRG exercises: gastrectomy].

    Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2008
    Same author

    [DRG practice: Frequently asked questions--questions from general practice].

    Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2007
    Same author

    [In Process Citation].

    Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2007

    Area of Science:

    • Biomedical Engineering
    • Hematology
    • Resuscitation Medicine

    Background:

    • Severe arterial blood loss necessitates effective oxygen-carrying resuscitation fluids.
    • Conventional cell-free hemoglobins face challenges with oxygen delivery and potential toxicity.
    • Developing safe and effective hemoglobin-based oxygen carriers (HBOCs) is a critical research area.

    Purpose of the Study:

    • To compare the efficacy of pyridoxylated stoma-free hemoglobin (SFH-PLP) and conventional stoma-free hemoglobin (SFH) in a canine model of hemorrhagic shock.
    • To evaluate the impact of these hemoglobin solutions on central hemodynamics and tissue oxygenation.
    • To assess the oxygen-unloading characteristics of SFH and SFH-PLP in vivo.

    Main Methods:

    • A canine model of severe arterial blood loss was established.

    Related Experiment Videos

  • Infusion of stoma-free hemoglobin (SFH) and pyridoxylated stoma-free hemoglobin (SFH-PLP) solutions.
  • Central hemodynamics were monitored post-infusion.
  • Skeletal muscle oxygenation was assessed using a multiwire platinum electrode.
  • Oxygen-hemoglobin dissociation curves were analyzed.

    • Main Results:

    • Both SFH and SFH-PLP effectively restored central hemodynamics without significant differences between groups.
    • No significant edema formation was observed despite large Ringer's lactate administration.
    • SFH-PLP infusion resulted in a rightward shift of the PO2-distribution curve, indicating improved skeletal muscle oxygenation.
    • Oxygen unloading from SFH was limited unless oxygen extraction exceeded 40%; SFH-PLP demonstrated improved oxygen unloading.

    Conclusions:

    • Pyridoxylated stoma-free hemoglobin (SFH-PLP) demonstrates superior tissue oxygenation compared to conventional SFH.
    • SFH-PLP shows potential as a short-term blood substitute due to enhanced oxygen delivery.
    • Further investigation into SFH-PLP is warranted for its application in resuscitation from hemorrhagic shock.