Visualization of dyes on thin-layer chromatography plates by means of the argon ion laser

A simple method for the detection of nanogram amounts of dyes by means of their fluorescence in the visible region of the spectrum is described. To stimulate the fluorescence, an argon ion laser with principal lines of 514.5 nm and 488.0 nm is used. The sensitivity of the technique allows the visualization of an amount of dye smaller than could be detected with either visible absorbance or ultraviolet stimulated fluorescence.