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Related Experiment Videos

A spot fluorometer for protein determination.

H G Zimmer, F Kiehl, V Neuhoff

    Hoppe-Seyler'S Zeitschrift Fur Physiologische Chemie
    |November 1, 1979
    PubMed
    Summary
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    A new apparatus enables in situ spot fluorometry for precise protein quantification. This method accurately measures fluorochrome-stained protein spots using Hoechst 2495.

    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Biotechnology

    Background:

    • Quantitative analysis of protein spots is crucial in biochemical research.
    • Existing methods for protein quantification can be time-consuming or require sample manipulation.
    • In situ measurement offers potential for streamlined analysis.

    Purpose of the Study:

    • To describe a novel apparatus for in situ spot fluorometry.
    • To enable quantitative evaluation of protein spots stained with fluorochromes.
    • To demonstrate the utility of Hoechst 2495 for this application.

    Main Methods:

    • Development of a specialized apparatus for in situ spot fluorometry.
    • Utilizing a fluorochrome (Hoechst 2495) for protein staining.
    • Performing quantitative measurements directly on protein spots.

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    Main Results:

    • The apparatus facilitates direct, in situ measurement of stained protein spots.
    • Quantitative evaluation of protein spot intensity is achievable.
    • The method demonstrates effectiveness with Hoechst 2495 stained proteins.

    Conclusions:

    • The described apparatus provides a viable tool for in situ spot fluorometry.
    • This technique allows for accurate quantitative assessment of protein spots.
    • The method is suitable for analyzing proteins stained with Hoechst 2495.