Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human glomerular cells in tissue culture.

A J Fish, A F Michael, R L Vernier

    Laboratory Investigation; a Journal of Technical Methods and Pathology
    |September 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Studies on chronic membranoproliferative glomerulonephritis with hypocomplementemia.

    The Journal of experimental medicine·2009
    Same author

    Cellular basis of diabetic nephropathy: III. In vitro GLUT1 mRNA expression and risk of diabetic nephropathy in type 1 diabetic patients.

    Diabetologia·2004
    Same author

    Back to medicine's future.

    Minnesota medicine·2000
    Same author

    Expression of the alpha6 chain of type IV collagen in glomerular basement membranes of healthy adult dogs.

    American journal of veterinary research·2000
    Same author

    New form of X-linked dominant hereditary nephritis in dogs.

    American journal of veterinary research·1999
    Same author

    Expression of mRNA for type IV collagen alpha1, alpha5 and alpha6 chains by cultured dermal fibroblasts from patients with X-linked Alport syndrome.

    Matrix biology : journal of the International Society for Matrix Biology·1998
    Same journal

    Changes in Three-Dimensional Intrahepatic Biliary Structures in Patients With Hepatobiliary Diseases Visualized Using Tissue-Clearing Methods.

    Laboratory investigation; a journal of technical methods and pathology·2026
    Same journal

    Genome-wide SNP-based Profiling of Loss of Heterozygosity Reveals Distinct Molecular Subgroup-specific Patterns in Gastrointestinal Stromal Tumors (GIST).

    Laboratory investigation; a journal of technical methods and pathology·2026
    Same journal

    AI-Assisted HER2 Scoring in Breast Cancer: Diagnostic Agreement and Understanding Discordance.

    Laboratory investigation; a journal of technical methods and pathology·2026
    Same journal

    Corrigendum to "POU2F3 in Small Cell Lung Cancer (SCLC): Diagnostic Utility in Neuroendocrine-Low/Negative SCLC and Discrimination From Other Thoracic Malignancies and Other Small Blue Round Cell Tumors" [Laboratory Investigation 2026;106(6):106124].

    Laboratory investigation; a journal of technical methods and pathology·2026
    Same journal

    Assessing the Effects of a 3D Pathology Tissue-Processing Workflow on Downstream Molecular Analyses.

    Laboratory investigation; a journal of technical methods and pathology·2026
    Same journal

    Transcription Factor Ets-1 Is a Central Regulator of Redox Balance and Liver Regeneration Through epidermal growth factor (EGF) and transforming growth factor-β (TGF-β) 1 Signaling.

    Laboratory investigation; a journal of technical methods and pathology·2026
    See all related articles

    Cultured human glomerular cells, distinct from fibroblasts, exhibit characteristics suggesting mesangial origin. These cells produce extracellular material sharing antigens with glomerular basement membranes and induce glomerulonephritis in monkeys.

    Area of Science:

    • Cell Biology
    • Nephrology
    • Immunology

    Background:

    • Understanding the cellular origins within the glomerulus is crucial for diagnosing and treating kidney diseases.
    • Human glomerular cells cultured in vitro provide a model for studying glomerular components and functions.

    Purpose of the Study:

    • To characterize cultured human glomerular cells morphologically and immunologically.
    • To investigate the potential origin and function of these cultured cells, particularly their relationship to mesangial cells.

    Main Methods:

    • Human glomeruli were explanted and cells were cultured and subcultivated.
    • Light and electron microscopy were used for morphological analysis.
    • Immunofluorescence microscopy and antibody absorption experiments were performed to assess antigenic properties.

    Related Experiment Videos

    Main Results:

    • Cultured cells were morphologically distinct from fibroblasts, showing extracellular material resembling basal lamina and intracellular microfilaments.
    • Extracellular material reacted with antiglomerular basement membrane antibody and shared antigenic components with the glomerular basement membrane.
    • Antibody against cultured cells localized to the mesangium and glomerular basement membrane in normal human kidneys and induced glomerulonephritis in monkeys.

    Conclusions:

    • The cultured glomerular cells possess characteristics, including extracellular matrix production and contractile elements, consistent with mesangial cell origin.
    • These findings support the hypothesis that the cultured cells are of mesangial origin and have implications for understanding glomerulonephritis pathogenesis.