Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High aflatoxin production on a chemically defined medium.

T V Reddy, L Viswanathan, T A Venkitasubramanian

    Applied Microbiology
    |September 1, 1971
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Red blood cell alloantibodies dictating solid organ transplant decisions: Two case reports.

    Asian journal of transfusion science·2026
    Same author

    D-- phenotype in a South Indian family: A multicentric approach to workup and management.

    Transfusion medicine (Oxford, England)·2025
    Same author

    Rh<sub>null</sub> phenotype in an Indian patient due to a novel c.1138 + 2 t > a mutation in the RHAG gene.

    Transfusion medicine (Oxford, England)·2024
    Same author

    Identification of SNPs in crucial starch biosynthesis genes in rice.

    Journal of genetics·2021
    Same author

    Novel antiproliferative flavonoids induce cell cycle arrest in human prostate cancer cell lines.

    Prostate cancer and prostatic diseases·2005
    Same author

    Free energy component analysis for drug design: a case study of HIV-1 protease-inhibitor binding.

    Journal of medicinal chemistry·2001

    This study found that Aspergillus parasiticus produces aflatoxins when provided with sucrose and asparagine. Removing asparagine drastically reduced toxin yields and altered the chemical profile of the fungal culture.

    Area of Science:

    • Microbiology
    • Mycology
    • Food Science

    Background:

    • Aflatoxins are toxic secondary metabolites produced by Aspergillus species.
    • Understanding the optimal conditions for aflatoxin production is crucial for food safety and agricultural practices.

    Purpose of the Study:

    • To investigate the influence of medium composition and pH on aflatoxin production by Aspergillus parasiticus ATCC 15517.
    • To identify key nutritional and environmental factors affecting aflatoxin biosynthesis.

    Main Methods:

    • Culturing Aspergillus parasiticus ATCC 15517 in a defined medium with varying sucrose and asparagine concentrations.
    • Analyzing aflatoxin yields using thin-layer chromatography (TLC).
    • Monitoring culture pH and optimizing inorganic salt concentrations (KH2PO4 and MgSO4·7H2O).

    Related Experiment Videos

    Main Results:

    • Aflatoxin yields ranged from 28 to 30 mg/100 ml in a medium with sucrose, asparagine, and salts.
    • Absence of asparagine led to a significant decrease in aflatoxin production and the appearance of novel fluorescent compounds.
    • Optimal initial pH for growth and toxin production was approximately 4.5.
    • Lower than standard concentrations of KH2PO4 and MgSO4·7H2O were found to be optimal.

    Conclusions:

    • Asparagine is essential for high-level aflatoxin production in Aspergillus parasiticus.
    • Culture pH and specific inorganic salt concentrations significantly impact aflatoxin biosynthesis.
    • Nutrient availability and environmental conditions can be manipulated to control aflatoxin formation.