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Updated: Aug 18, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
Structure-function relationship: biological activities of the lipopolysaccharides and lipid A from Vibrio cholerae
Abstract:
The biological activities of lipopolysaccharides and the lipid A moiety from five different strains of Vibrio cholerae have been investigated. Lipid A was biologically the most active component of lipopolysaccharides from these bacteria. Immunization of rabbits with lipid A from Inaba (66/64) strain led to the production of specific antibodies to lipid A. On fractionation of the immunoglobulins with Sephadex G-200 column chromatography, antibodies against lipid A were detected in the IgM and IgG fractions.
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