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Related Experiment Videos

Calcium distribution in Amoeba proteus.

R D Prusch, J A Hannafin

    The Journal of General Physiology
    |October 1, 1979
    PubMed
    Summary

    Cellular calcium distribution in Amoeba proteus was studied. Increased external calcium leads to influx, with most calcium binding to the cell surface, not the cytoplasm.

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    Area of Science:

    • Cell Biology
    • Biophysics

    Background:

    • Cellular calcium homeostasis is crucial for various biological processes.
    • Understanding calcium distribution in single-celled organisms like Amoeba proteus provides insights into fundamental cellular mechanisms.

    Purpose of the Study:

    • To investigate the distribution and exchange of calcium ions (Ca++) in Amoeba proteus.
    • To determine how external calcium concentration and pH affect cellular calcium levels and binding.

    Main Methods:

    • Measurement of total cellular calcium under varying external Ca++ concentrations.
    • Utilizing the radioisotope 45Ca to study steady-state calcium exchange.
    • Employing lanthanum (La+++) to assess the location of exchanged calcium.

    Main Results:

    • Amoeba proteus maintains a steady-state cellular calcium level within 3 hours of altered external Ca++ concentration.
    • At external Ca++ levels below 10 mM, cellular calcium concentration exceeds that of the external solution.
    • An initial rapid exchange of 18% of total cellular calcium was observed, primarily associated with the cell surface and removable by La+++.
    • Increased external Ca++ or pH enhanced the amount of exchangeable cell-surface calcium.

    Conclusions:

    • A significant portion of cellular calcium in Amoeba proteus is associated with the cell surface, potentially through co-ion binding or surface sites.
    • Cytoplasmic calcium ion activity is maintained at very low levels.
    • The cell surface plays a key role in regulating calcium ion influx and distribution.

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