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Related Experiment Videos

Histone kinase and cell division.

A R Pawse, M G Ord, L A Stocken

    The Biochemical Journal
    |May 1, 1971
    PubMed
    Summary
    This summary is machine-generated.

    Histone F1 phosphokinase activity rises in regenerating rat liver, but gamma-irradiation significantly reduces it. This suggests irradiation impacts DNA synthesis regulation during liver regeneration.

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    Area of Science:

    • Molecular Biology
    • Cellular Biology
    • Biochemistry

    Background:

    • Liver regeneration involves complex regulatory processes, including DNA synthesis.
    • Histone modifications, such as phosphorylation, play crucial roles in regulating DNA synthesis and cell cycle progression.
    • Histone F1 phosphokinase is an enzyme involved in the phosphorylation of histone F1.

    Purpose of the Study:

    • To investigate the changes in histone F1 phosphokinase activity during liver regeneration.
    • To determine the effect of gamma-irradiation on histone F1 phosphokinase levels in regenerating liver.
    • To characterize the enzymatic properties of histone F1 kinase and phosphatase preparations.

    Main Methods:

    • Partial hepatectomy was performed on rats to induce liver regeneration.

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  • Gamma-irradiation was administered at a specific time point during the G1 phase.
  • Histone F1 phosphokinase and phosphatase activities were measured in liver extracts at different time points post-hepatectomy.
  • Phosphate incorporation into histone F1 subclasses was analyzed using radioactive isotopes.

    • Main Results:

    • Histone F1 phosphokinase activity increased during the early phase of DNA synthesis following partial hepatectomy, suggesting de novo enzyme synthesis.
    • A dose of 500rd gamma-irradiation given early in G1 reduced histone F1 phosphokinase levels by 60-70% at 22 hours post-hepatectomy.
    • Enzyme preparations contained both histone F1 kinase and phosphatase activities, influencing net phosphate uptake and incorporation.
    • All four histone F1 subclasses were capable of accepting phosphate from ATP.
    • Crude enzyme preparations showed differential phosphate transfer based on the initial phosphate content of histone F1.

    Conclusions:

    • Histone F1 phosphokinase activity is upregulated during liver regeneration and is sensitive to gamma-irradiation.
    • Gamma-irradiation likely interferes with the regulation of DNA synthesis during liver regeneration by inhibiting histone F1 phosphokinase.
    • The interplay between histone F1 kinase and phosphatase activities is critical for accurate assessment of histone phosphorylation dynamics.