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Related Experiment Videos

Isolation of filaments from brain.

M L Shelanski, S Albert, G H DeVries

    Science (New York, N.Y.)
    |December 17, 1971
    PubMed
    Summary

    Researchers isolated 90-angstrom protein filaments from bovine brain white matter. These stable filaments, primarily protein, have a subunit molecular weight of 60,000 and do not bind colchicine or nucleotides.

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    Area of Science:

    • Neuroscience
    • Biochemistry
    • Cell Biology

    Background:

    • White matter contains various protein structures crucial for neuronal function.
    • Understanding the composition of these structures aids in comprehending neurological processes.

    Purpose of the Study:

    • To develop a method for isolating specific protein filaments from bovine brain white matter.
    • To characterize the biochemical and biophysical properties of these isolated filaments.

    Main Methods:

    • Isolation of filaments using ultracentrifugation and density gradient fractionation of myelinated axons.
    • Assessment of filament stability across temperature and ionic strength variations.
    • Enzymatic digestion assays (ribonuclease, deoxyribonuclease) and molecular weight determination.

    Main Results:

    • Successfully isolated a fraction enriched in two types of 90-angstrom diameter filament bundles.
    • Filaments demonstrated high stability across wide temperature and ionic strength ranges.
    • Filaments are primarily protein (subunit MW ~60,000), resistant to nucleases, and do not interact with microtubule protein, colchicine, or nucleotides.

    Conclusions:

    • A novel method for isolating specific protein filaments from brain white matter was established.
    • The isolated filaments represent a distinct protein component of white matter with unique stability and biochemical properties.
    • These findings contribute to the understanding of white matter protein composition and potential functions.

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