Jove
Visualize
Contact Us

Related Experiment Videos

X-ray diffraction from the side-by-side model of DNA.

R J Greenall, W J Pigram, W Fuller

    Nature
    |December 20, 1979
    PubMed
    Summary

    Novel side-by-side DNA structures were proposed to solve unwinding problems. However, these models show significant discrepancies with observed B-DNA diffraction patterns, suggesting the Watson-Crick model remains superior.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Advances in Cell Signaling Pathways: A Comprehensive Review

    Journal of Cellular Biology·2024
    Same author

    Novel Approaches to Tissue Engineering and Regenerative Medicine

    Nature Methods·2023
    Same author

    Understanding Molecular Mechanisms in Disease Progression

    Cell Reports·2023
    Same author

    Genomic Profiling Reveals New Biomarkers for Early Diagnosis

    Nature Genetics·2023
    Same author

    CRISPR-Based Screening Identifies Key Regulators of Cell Growth

    Cell Reports·2022
    Same author

    Structural Insights into Membrane Protein Function

    Journal of Cellular Biology·2022
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies

    Area of Science:

    • Molecular Biology
    • Structural Biology
    • Biophysics

    Background:

    • The Watson-Crick DNA model presents topological challenges for strand unwinding during replication.
    • Novel side-by-side (SBS) DNA structures have been proposed to address these unwinding issues.

    Purpose of the Study:

    • To evaluate the structural validity of proposed side-by-side DNA models.
    • To compare the diffraction patterns of SBS models with the observed B-DNA diffraction pattern.

    Main Methods:

    • Analysis of X-ray diffraction data for B-DNA.
    • Computational modeling and comparison of diffraction patterns for SBS DNA structures and the Watson-Crick model.

    Main Results:

    • Unacceptable discrepancies were found between the observed B-DNA diffraction pattern and that calculated for the original SBS structure.
    • A modified SBS model showed some improvement but remained inferior to the Watson-Crick model in agreement with experimental data.

    Conclusions:

    • The original and modified side-by-side DNA models do not fully explain the observed B-DNA diffraction patterns.
    • The classical Watson-Crick DNA model provides a superior explanation for the experimental diffraction data.

    Related Experiment Videos