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[Procedure for isolating nisin from Streptococcus lactis].
Prikladnaia Biokhimiia I Mikrobiologiia
|September 1, 1979
Summary
This study details a method for extracting the antibiotic nisin from Streptococcus lactis using acetic acid. The nisin concentrate is spray-dried, but loses potency over a year of refrigeration.
Area of Science:
- Microbiology
- Biochemistry
- Food Science
Context:
- Nisin is a bacteriocin produced by Streptococcus lactis.
- Industrial production of nisin requires efficient extraction and preservation methods.
Purpose:
- To describe a method for extracting and processing nisin from Streptococcus lactis.
- To evaluate the stability of the nisin preparation during storage.
Summary:
- A method involving 50% acetic acid extraction was used to obtain nisin from Streptococcus lactis.
- The extracted nisin concentrate was dried using an Anhydro spray drier.
- The nisin preparation showed a 20-25% inactivation after one year of refrigerated storage.
Impact:
- Provides a detailed extraction protocol for nisin production.
- Highlights the stability limitations of the nisin preparation under refrigeration.
- Informs further research on nisin preservation techniques for extended shelf-life.