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Related Experiment Videos

Lymphocyte-mediated cellular immunity in histoplasmosis.

D H Howard, V Otto, R K Gupta

    Infection and Immunity
    |November 1, 1971
    PubMed
    Summary

    Lymphocytes from immunized mice prevent Histoplasma capsulatum growth in macrophages. This immune cell-mediated restriction is lost when cells are cultured long-term, highlighting the crucial role of lymphocytes in controlling fungal infections.

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    Area of Science:

    • Immunology
    • Mycology
    • Cell Biology

    Background:

    • Freshly harvested mononuclear phagocytes from immunized animals inhibit intracellular Histoplasma capsulatum growth.
    • This inhibitory effect is lost in phagocytes cultured for 48 hours.
    • The cellular mechanisms underlying this transient immune response require elucidation.

    Purpose of the Study:

    • To identify the specific immune cell responsible for restricting intracellular Histoplasma capsulatum growth in vivo.
    • To investigate the role of lymphocytes in mediating this antifungal activity.
    • To understand the conditions under which this immune-mediated restriction is maintained.

    Main Methods:

    • Harvesting mononuclear phagocytes and lymphocytes from immunized mice.
    • Culturing cells in vitro to assess Histoplasma capsulatum growth.
    • Utilizing partially purified lymphocyte populations in co-culture experiments with normal macrophages.

    Main Results:

    • Mononuclear phagocytes from immunized mice restrict fungal growth initially.
    • Lymphocytes isolated from the peritoneal cavity of immunized mice were identified as key mediators of this growth restriction.
    • Partially purified lymphocytes suppressed intracellular Histoplasma capsulatum growth in normal mouse macrophages in cell culture.

    Conclusions:

    • Lymphocytes are critical mediators of intracellular growth restriction of Histoplasma capsulatum in immunized hosts.
    • The presence and interaction of lymphocytes are essential for maintaining the antifungal activity of phagocytes.
    • This study elucidates a lymphocyte-dependent mechanism for controlling fungal infections at the cellular level.

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