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Intrarenal Injection of Escherichia coli in a Rat Model of Pyelonephritis
Published on: July 18, 2017
Effect of diuresis on Staphylococcus aureus kidney infections in mice
Abstract:
In the Cornett strain of mice, water diuresis did not prevent hematogenous production of pyelonephritis by Staphylococcus aureus. Increased fluid intake did not affect the numbers of organisms deposited in the kidneys or the rate of growth during the first 4 hr after inoculation. Drinking the glucose solution did not enhance bacterial proliferation within the renal parenchyma. Subcutaneous injection of saline to supplement for interruption of drinking after inoculation reduced the numbers of organisms recovered in the kidneys but not sufficiently to prevent production of pyelonephritis. Incorporating penicillin as a marker indicated that fluids administered by subcutaneous injections were rapidly delivered to the kidneys. Combining diuresis with treatment did not influence the rapidity of delivery of antimicrobial to the kidneys or the length of time that it was present in the renal homogenate.
Insights
Water diuresis did not prevent Staphylococcus aureus pyelonephritis in mice. While saline injections reduced bacterial load, they did not fully prevent infection, and fluid administration did not alter antimicrobial delivery to kidneys.
Area of Science:
- Nephrology
- Infectious Diseases
- Microbiology
Background:
- Pyelonephritis is a serious kidney infection often caused by Staphylococcus aureus.
- Understanding host-pathogen interactions and the impact of fluid balance is crucial for treatment strategies.
Purpose of the Study:
- To investigate the efficacy of water diuresis in preventing Staphylococcus aureus pyelonephritis in a mouse model.
- To assess the effect of fluid intake and administration on bacterial growth and antimicrobial delivery in the kidneys.
Main Methods:
- Hematogenous inoculation of Staphylococcus aureus in Cornett strain mice.
- Administration of water, glucose solution, or saline via drinking or subcutaneous injection.
- Quantification of bacterial load in kidneys and assessment of pyelonephritis development.
- Use of penicillin as a marker to track fluid and antimicrobial delivery to renal tissue.
Main Results:
- Water diuresis did not prevent the development of pyelonephritis.
- Increased fluid intake did not alter initial bacterial deposition or growth rates in the kidneys.
- Subcutaneous saline reduced bacterial numbers but was insufficient to prevent pyelonephritis.
- Fluids administered subcutaneously were rapidly delivered to the kidneys, but this did not affect antimicrobial delivery or persistence.
Conclusions:
- Water diuresis is ineffective in preventing Staphylococcus aureus pyelonephritis.
- Fluid administration strategies, including subcutaneous injections, have limited impact on preventing infection progression or enhancing antimicrobial efficacy in this model.
