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Related Experiment Videos

Nuclear acidic protein changes during differentiation in Physarum polycephalum.

W M LeStourgeon, H P Rusch

    Science (New York, N.Y.)
    |December 17, 1971
    PubMed
    Summary

    Acidic nuclear phosphoproteins in Physarum polycephalum are synthesized rapidly after mitosis and show changes during differentiation. These proteins may play a role in controlling gene activity.

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    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • Acidic nuclear phosphoproteins are crucial components of eukaryotic nuclei.
    • Understanding their role in cell cycle and differentiation is key to cell regulation.

    Purpose of the Study:

    • To isolate and characterize acidic nuclear phosphoproteins in Physarum polycephalum.
    • To investigate the changes in these proteins during the mitotic cycle and differentiation.

    Main Methods:

    • Isolation of acidic nuclear phosphoproteins throughout the cell cycle and differentiation.
    • Electrophoretic analysis to determine protein profiles and molecular weights.
    • Radioactive labeling with [(32)P]orthophosphate to assess synthesis and phosphorylation.

    Main Results:

    • Proteins isolated range from 34,000 to 88,000 molecular weight.
    • Electrophoretic profiles are stable during the mitotic cycle but change during differentiation.
    • Rapid synthesis post-mitosis and increased phosphate incorporation after DNA synthesis observed.

    Conclusions:

    • Acidic nuclear phosphoproteins exhibit dynamic changes during differentiation.
    • These proteins are actively synthesized and phosphorylated.
    • Potential role in the regulation of gene activity suggested.

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