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Updated: Aug 1, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Inhibition of D-glyceraldehyde-3-phosphate dehydrogenase by ATP and quinaldate
Abstract:
The inhibition of D-glyceraldehyde-3-phosphate dehydrogenase by ATP is of purely mixed type with respect to NAD (Ki=4.9 mM), purely uncompetitive with respect to D-glyceraldehyde-3-phosphate (Ki=9.4 mM) and partially uncompetitive with respect to inorganic phosphate (Ki=6.0 mM). Quinaldate is a purely mixed type inhibitor with respect to both NAD (Ki==10.0 mM) and D-glyceraldehyde-3-phosphate (Ki=15.3 mM), whereas purely non-competitive with respect to inorganic phosphate (Ki=11.0 mM). In the presence of quinaldate a lag period is observed in the time course of enzyme reaction. The duration of this lag period depends on both quinaldate and substrate concentrations.
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