Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Rheumatic Heart Disease III: Medical Management01:21

Rheumatic Heart Disease III: Medical Management

268
Rheumatic heart disease (RHD) management can be divided into two main strategies: prevention and long-term management.Primary PreventionPrimary prevention focuses on timely diagnosis and management of group A streptococcal pharyngitis to prevent acute rheumatic fever. The most widely used antibiotic for treating this condition is intramuscular benzathine penicillin G.Acute Rheumatic Fever TreatmentThe primary treatment goal for a patient diagnosed with acute rheumatic fever is to suppress the...
268
Rheumatic Heart Disease I: Introduction01:23

Rheumatic Heart Disease I: Introduction

415
Rheumatic heart disease or RHD is a chronic condition that results from rheumatic fever, causing permanent damage to the heart valves.Etiology and Risk FactorsIt primarily arises from rheumatic fever, an inflammatory disease that can develop after untreated or inadequately treated group A streptococcal (GAS) pharyngitis. Streptococcus spreads through direct contact with oral or respiratory secretions. While the bacteria are the causative agents, factors like malnutrition, overcrowding, poor...
415
Bacterial Phylum Spirochaetes01:30

Bacterial Phylum Spirochaetes

548
Spirochetes, unique bacteria in the phylum Spirochaetes, are gram-negative, motile, tightly coiled, slender, and flexible. They inhabit aquatic sediments and animals, with some causing diseases like syphilis. Spirochetes are classified into eight genera based on habitat, pathogenicity, phylogeny, and characteristics.Their distinctive motility arises from endoflagella, located within the cell’s periplasm. These endoflagella anchor at the cell poles and extend along the cell length, encased...
548
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

1.2K
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
1.2K
Bacterial Phylum Actinobacteria01:30

Bacterial Phylum Actinobacteria

582
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
582
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

2.6K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prospective study of the additional benefit of plexus magnetic resonance imaging in the diagnosis of chronic inflammatory demyelinating polyneuropathy.

European journal of neurology·2019
Same author

Does Type of Substratum Affects Chironomid Larvae Assemblage Composition? A Study in a River Catchment in Northern Patagonia, Argentina.

Neotropical entomology·2016
Same author

Isolated tumefactive demyelinating lesions: diagnosis and long-term evolution of 16 patients in a multicentric study.

Journal of neurology·2015
Same author

Measurement of the bottom-strange meson mixing phase in the full CDF data set.

Physical review letters·2012
Same author

Precision top-quark mass measurement at CDF.

Physical review letters·2012
Same author

Measurement of Bs0→Ds(*)+Ds(*)- branching ratios.

Physical review letters·2012

Related Experiment Video

Updated: Jan 10, 2026

Bacterial Phylum Spirochaetes
01:30

Bacterial Phylum Spirochaetes

Published on: June 12, 2025

548

Fibronectin: a chromatin-associated protein?

L Zardi, A Siri, B Carnemolla

    Cell
    |November 1, 1979
    PubMed
    Summary

    Human cultured fibroblasts contain fibronectin, a DNA-binding serum protein. This protein, fibronectin, constitutes 0.7% of total cell protein and binds DNA with partial specificity.

    Area of Science:

    • Molecular Biology
    • Biochemistry

    Background:

    • Chromatin preparations from human cultured fibroblasts were previously found to contain a single homologous serum protein.
    • The identity and function of this protein within the cell nucleus remained to be fully elucidated.

    Purpose of the Study:

    • To identify the homologous serum protein in human fibroblast chromatin.
    • To characterize the DNA-binding properties and specificity of fibronectin.
    • To determine the abundance of fibronectin in chromatin and whole cells.

    Main Methods:

    • Immunological identity and physicochemical property analysis were used for protein identification.
    • Radioimmunoassay (RIA) was employed to quantify fibronectin levels.
    • Nitrocellulose filter assays and equilibrium competition experiments assessed DNA-binding affinity and specificity.

    More Related Videos

    Development of Antibiotic Resistance
    01:30

    Development of Antibiotic Resistance

    Published on: June 12, 2025

    1.2K
    Bacterial Phylum Actinobacteria
    01:30

    Bacterial Phylum Actinobacteria

    Published on: June 6, 2025

    582

    Related Experiment Videos

    Last Updated: Jan 10, 2026

    Bacterial Phylum Spirochaetes
    01:30

    Bacterial Phylum Spirochaetes

    Published on: June 12, 2025

    548
    Development of Antibiotic Resistance
    01:30

    Development of Antibiotic Resistance

    Published on: June 12, 2025

    1.2K
    Bacterial Phylum Actinobacteria
    01:30

    Bacterial Phylum Actinobacteria

    Published on: June 6, 2025

    582
  • DNA-affinity chromatography was utilized for fibronectin isolation.

    • Main Results:

    • The homologous serum protein in chromatin was identified as fibronectin.
    • Fibronectin constitutes approximately 0.7% of total protein in both chromatin and whole fibroblasts.
    • Fibronectin demonstrated DNA-binding activity with an equilibrium constant of 4.6 x 10(-6) M.
    • Fibronectin exhibits partial specificity for DNA, requiring a minimum polymer length of 12-18 nucleotides for effective binding.

    Conclusions:

    • Fibronectin is a DNA-binding protein found in human fibroblast chromatin.
    • Fibronectin appears to be a normal constituent of isolated chromatin, though direct evidence as a nonhistone chromosomal protein is lacking.
    • Fibronectin's DNA-binding properties suggest a potential role in nuclear processes.