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Ox kidney uricase: a new method of purification
The Italian Journal of Biochemistry
|July 1, 1979
Summary
Researchers purified uricase (urate oxidase) from ox kidneys using a novel procedure. This method achieved a 3,800-fold purification, yielding a highly pure enzyme suitable for further biochemical applications.
Area of Science:
- Biochemistry
- Enzymology
Background:
- Uricase (urate oxidase) is an enzyme that catalyzes the oxidation of uric acid.
- Efficient purification of active uricase is crucial for biochemical and therapeutic applications.
Purpose of the Study:
- To develop an effective purification protocol for uricase from ox kidney sources.
- To achieve a high degree of enzyme purity and specific activity.
Main Methods:
- Extraction of uricase from ox kidneys using n-butanol.
- Purification employing gel filtration chromatography.
- Purification utilizing ion-exchange chromatography.
Main Results:
- A highly purified uricase enzyme was obtained.
- The enzyme exhibited a specific activity of 50 units/mg.
- This represents a 3,800-fold increase in purification.
- Gel electrophoresis indicated near-homogeneity of the final enzyme preparation.
Conclusions:
- The developed purification procedure is effective for isolating highly pure and active uricase.
- The method offers a significant fold purification, making the enzyme accessible for research.