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F antigen. III. Immunological and biological properties
Pathologia Et Microbiologia
|January 1, 1975
Summary
Mice can generate antibodies against F antigens from other species. A small, hapten-like fragment of the F protein appears responsible for its widespread cross-reactivity and detection in liver disease.
Area of Science:
- Immunology
- Biochemistry
- Molecular Biology
Background:
- F antigens exhibit distinct isoforms in different mouse strains (A2G and CBA).
- Inbred mice strains can develop anti-F antibodies when exposed to xenogeneic F antigens.
- F antigen presence is noted early in human development and typically absent in serum unless liver disease is present.
Purpose of the Study:
- To elucidate the immunogenicity and cross-reactivity of F antigen isoforms.
- To characterize the role of F antigen fragments in antibody interactions.
- To establish a sensitive method for F antigen quantification.
Main Methods:
- Immunoelectrophoresis was employed for sensitive antigen quantitation.
- Studies on antibody-antigen precipitation and inhibition assays were conducted.
- Analysis of F antigen behavior under denaturing conditions.
Main Results:
- The immunogenicity of F antigen isoforms from A2G and CBA mouse strains was detailed.
- A small fragment, released under denaturing conditions, inhibited antigen-antibody precipitation.
- This fragment demonstrated haptenic properties, suggesting its role in cross-reactivity.
Conclusions:
- The F protein acts as a carrier for a small, haptenic fragment responsible for universal cross-reactivity.
- The findings provide insights into F antigen structure, function, and immunologic interactions.
- Sensitive detection of F antigen may be relevant in diagnosing acute liver disease.