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Human lymphoblastoid cells in long-term cultures: electron microscopic study.
Neoplasma
|January 1, 1979
Summary
Lymphoblastoid cell lines (LCL) derived from leukemia patients maintained their primitive lymphoblastic morphology. These cell lines showed stability over time, cryopreservation, and lacked signs of viral contamination or differentiation.
Area of Science:
- Hematology
- Cell Biology
- Electron Microscopy
Background:
- Lymphoblastoid cell lines (LCL) are crucial tools in leukemia research.
- Understanding the stability and characteristics of LCLs is vital for reliable experimental outcomes.
Purpose of the Study:
- To investigate the fine structure and stability of LCLs derived from acute myelosis and undifferentiated acute leukemia patients.
- To assess morphological changes over extended cultivation, cryopreservation, and recultivation.
Main Methods:
- Electron microscopy was used for repeated fine structure analysis of LCLs.
- Cells were studied after prolonged cultivation, freezing and thawing, and recultivation.
Main Results:
- The majority of cells in all LCLs consistently exhibited a primitive lymphoblastic morphology.
- Cell morphology remained stable despite extended cultivation, cryopreservation, and recultivation.
- No evidence of viral contamination or differentiation into mature cell types was observed.
Conclusions:
- Leukemia-derived LCLs maintain a stable lymphoblastic phenotype.
- These findings support the utility of LCLs in long-term studies without significant morphological alteration.