Affinity chromatography of human serum proteins using matrix bound lectins from Viscum album and Vicia faba

The D-galactose specific lectin from Viscum album and the D-mannose specific lectin from Vicia faba react with serum proteins which contain the corresponding carboyhydrate moieties. By affinity chromatography of human serum using the combination of insolubilized lectins coupled to Sepharose it is possible to fractionate serum proteins in 3 groups: 1. proteins which react with both of the lectins; 2. proteins which react only with one of the lectins; 3. proteins which do not display any affinity for one of the two lectins.