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Related Concept Videos

Mucosal Barrier of the Stomach01:25

Mucosal Barrier of the Stomach

The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
Gastritis-II: Pathophysiology01:17

Gastritis-II: Pathophysiology

Gastritis is marked by disruption of the mucosal barrier that usually protects the stomach tissue from digestive juices and manifests in acute and chronic forms.
In acute gastritis, the gastric mucosa becomes swollen and red and undergoes superficial erosion. Superficial ulceration may lead to bleeding.
In chronic gastritis, persistent or repeated insults lead to chronic inflammatory changes and, eventually, thinning or atrophy of the gastric tissue.
Gastritis can stem from various causes, each...

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Isolation of parietal cells from guinea-pig gastric mucosa and the immunological characterization of their antigenic

D P Jewell, V N Katiyar, C Rees

    Gut
    |August 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a method to isolate guinea pig parietal cells using collagenase. This technique yielded a high percentage of viable cells and enabled the production of antibodies against key parietal cell antigens.

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    Area of Science:

    • Gastroenterology
    • Cell Biology
    • Immunology

    Background:

    • Parietal cells are crucial for gastric acid secretion.
    • Isolation of pure, viable parietal cells is essential for studying their function and associated diseases.
    • Previous methods for parietal cell isolation had limitations.

    Purpose of the Study:

    • To establish a reliable method for isolating viable guinea pig parietal cells.
    • To characterize the isolated parietal cells.
    • To generate antibodies against parietal cell-specific antigens for further research.

    Main Methods:

    • Enzymatic digestion of gastric mucosa with collagenase.
    • Cell viability assessment using standard assays.
    • Identification of parietal cells via morphology (light and electron microscopy), neutral red uptake, immunofluorescence, and carbonic anhydrase activity.
    • Antibody production by immunizing rabbits with isolated parietal cells and their fractions.

    Main Results:

    • A method yielding 70-80% pure parietal cells was established.
    • Initial cell viability was high (~80%) but decreased over one hour.
    • Four distinct antibodies were generated against microsomal fractions, plasma/nuclear membranes, soluble proteins, and intrinsic factor.
    • The antibody against the microsomal fraction demonstrated significant species specificity.

    Conclusions:

    • Enzymatic isolation with collagenase provides a viable method for obtaining enriched parietal cell suspensions.
    • The generated antibodies are valuable tools for studying parietal cell antigens and their role in conditions like pernicious anemia.
    • The species specificity of antibodies is an important consideration for immunological studies.