Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Split-operon control of a prophage gene.

L E Bertani

    Proceedings of the National Academy of Sciences of the United States of America
    |February 1, 1970
    PubMed
    Summary
    This summary is machine-generated.

    Bacteriophage P2 integration and excision require the int function. This int function is constitutively expressed by superinfecting phages but not by prophages, suggesting it

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Erich Walther Six (1926–2014): discoverer of the satellite phage P4.

    Virology·2015
    Same author

    Physical and genetic characterization of the genome of Magnetospirillum magnetotacticum, strain MS-1.

    Gene·2001
    Same author

    Paleoproterozoic snowball earth: extreme climatic and geochemical global change and its biological consequences.

    Proceedings of the National Academy of Sciences of the United States of America·2000
    Same author

    Evidence for two types of subunits in the bacterioferritin of Magnetospirillum magnetotacticum.

    Gene·1997
    Same author

    The isolation and characterization of the gene (dfr1) encoding dihydrofolate reductase (DHFR) in Schizosaccharomyces pombe.

    Gene·1994
    Same author

    Control of prophage integration and excision in bacteriophage P2: nucleotide sequences of the int gene and att sites.

    Gene·1989
    Same journal

    In This Issue.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Long-term cultural continuity across the Neanderthal-modern human sequence at Üçağızlı II Cave, northern Levant.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Dolphins use names to remember whom to avoid.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Retraction for Shaked and Frenkel, Curiouser and curiouser: Meningeal lymphoid structures in the aging brain.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Small but mighty: The outsized role of small water bodies in the global carbon cycle.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Functional traits produce conditional outcomes in different community contexts.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Bacteriophage P2 integration and excision are critical processes for its life cycle.
    • These events are known to depend on a specific phage gene product, termed the integrase (int) function.

    Purpose of the Study:

    • To investigate the regulation and expression of the P2 int function during integration and excision.
    • To determine the role of phage repressor in controlling int function expression.

    Main Methods:

    • Experiments involving superinfecting bacteriophage P2 in both immune and non-immune hosts.
    • Analysis of prophage P2 excision in derepressed lysogens with and without superinfecting phage providing int function.

    Main Results:

    Related Experiment Videos

  • Bacteriophage P2 can efficiently integrate into a host chromosome even in an immune host if the int function is provided.
  • Prophage P2 cannot be excised from the host chromosome in a derepressed lysogen unless the int function is supplied by a superinfecting phage.
  • The int function is constitutively expressed by superinfecting phages but not by the prophage, even when phage repressor is absent.

    • Conclusions:

    • The P2 int function is constitutively expressed by superinfecting phages.
    • Prophage integration appears to disrupt a constitutive operon, leading to the lack of int function expression from the prophage.
    • Phage repressor does not control the expression of the P2 int function.