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Specific nucleolar and nucleoplasmic RNA polymerases.

R G Roeder, W J Rutter

    Proceedings of the National Academy of Sciences of the United States of America
    |March 1, 1970
    PubMed
    Summary
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    Rat liver nuclei contain distinct DNA-dependent RNA polymerases. These enzymes, RNA polymerase I and II, are localized in specific nuclear regions, suggesting roles in genetic transcription regulation.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Background:

    • DNA-dependent RNA polymerase activity was previously identified in rat liver nuclei.
    • Two major chromatographically distinct enzymatic species, RNA polymerase I and II, were reported in whole nuclei.

    Purpose of the Study:

    • To solubilize and purify DNA-dependent RNA polymerases from rat liver nuclei.
    • To investigate the subnuclear localization of these RNA polymerases.

    Main Methods:

    • Solubilization and chromatographic purification of RNA polymerases from whole rat liver nuclei.
    • Subfractionation of nuclei into nucleolar and nucleoplasmic fractions.
    • Analysis of RNA polymerase activity in different nuclear fractions.

    Main Results:

    Related Experiment Videos

    • Purification yielded two major RNA polymerase species (I and II) from whole nuclei.
    • Nucleoli were enriched in RNA polymerase I.
    • The nucleoplasmic fraction was significantly enriched in RNA polymerase II, with a minor third peak also detected.

    Conclusions:

    • RNA polymerases are specifically localized within the rat liver nucleus.
    • This specific localization suggests distinct roles for RNA polymerases in the regulation of genetic transcription.