Electrophoretic analysis of hemoglobin and isozymes in individual vertebrate cells

Electrophoretic patterns of enzymes in single vertebrate erythrocytes may be visualized with the use of capillary tube polyacrylamide columns and stains which amplify the enzymatic activity. The following points have thereby been demonstrated:1. Peripheral blood of metamorphosing bullfrogs (Rana catesbeiana) contains two molecular forms of hemoglobin: embryonic (tadpole) and adult (frog). Only one of these forms is found in each erythrocyte.2. Human umbilical cord blood contains fetal and adult hemoglobins; both forms may be found in individual erythrocytes, in varying proportions.3. Both lactate dehydrogenase isozymes found in whole blood of bullfrogs and humans are found in individual erythrocytes, but the proportions differ from cell to cell.