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Surface structure of Uukuniemi virus.

C H von Bonsdorff, R Pettersson

    Journal of Virology
    |November 1, 1975
    PubMed
    Summary

    Uukuniemi virus particles are approximately 95 nm in diameter, featuring a membrane with surface projections. Proteolytic enzyme treatment reveals hollow cylindrical units, suggesting an icosahedral surface lattice structure.

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    Area of Science:

    • Virology
    • Structural Biology
    • Cell Biology

    Background:

    • Uukuniemi virus is an enveloped virus studied for its structural properties.
    • Understanding viral structure is crucial for comprehending viral assembly and infection mechanisms.

    Purpose of the Study:

    • To elucidate the detailed structure of the Uukuniemi virus particle.
    • To characterize the surface projections and underlying membrane structure.

    Main Methods:

    • Electron microscopy techniques including negative staining, thin sectioning, and freeze-etching.
    • Proteolytic digestion using thermolysin to analyze surface protein composition.

    Main Results:

    • Uukuniemi virus particles are spherical, approximately 95 nm in diameter, with a 5-nm membrane and 8-10 nm surface projections.
    • Surface projections are composed of two polypeptide species, forming hollow cylindrical units (10-12 nm) with a 5-nm central cavity.
    • Enzyme treatment resulted in smooth, deformable particles, indicating the removal of surface structures.
    • The surface lattice appears to be an icosahedral arrangement (T=12, P=3) of penton-hexon clusters.
    • The membrane is likely a lipid bilayer, with nucleoprotein located beneath it.

    Conclusions:

    • The Uukuniemi virus possesses a complex surface structure composed of polypeptide units arranged in an icosahedral lattice.
    • The membrane structure and the arrangement of surface glycoproteins provide insights into viral assembly and stability.

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