Carbon-13 Fourier transform nuclear magnetic resonance. II. Ribonuclease

High-resolution Fourier transform nuclear magnetic resonance was used to observe the natural abundance (13)C spectrum of a 0.02 M solution of bovine pancreatic ribonuclease A. Peaks assignable to only three carbons were easily observed after 10 hr of signal averaging. A number of tentative assignments were made. As expected, the (13)C spectrum was appreciably richer in detail than the corresponding proton spectrum. Natural abundance (13)C Fourier transform nmr appears to be a practical tool for the study of biopolymers in solution.