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Characterization of a Friend virus-replicating target cell
Experimental Hematology
|October 1, 1979
Summary
This study identifies FV-replicating cells as erythroid precursors, distinct from BFU-E and partially or non-identical to CFU-E. These cells form primary erythroleukemic lesions in spleen colonies.
Area of Science:
- Hematology
- Cell Biology
- Virology
Background:
- Erythroid precursor cells are crucial for red blood cell production.
- Friend వైరస్ (FV) infection affects hematopoietic stem cells.
- Understanding FV target cells is key to erythroleukemia pathogenesis.
Purpose of the Study:
- To characterize the FV-replicating target cell in erythroid precursors.
- To differentiate FV target cells from known erythroid progenitor populations (BFU-E, CFU-E).
- To investigate the role of FV-infected target cells in erythroleukemia development.
Main Methods:
- Cell response analysis to bleeding and hypertransfusion.
- Comparison of cell cycle kinetics and model velocities.
- Assessment of sensitivity to AMD (an inhibitor).
- Quantitative analysis of cell populations in hematopoietic organs.
- Spleen colony assay to evaluate erythroleukemic potential.
Main Results:
- FV-replicating cells are erythroid precursors, responding to physiological stimuli.
- FV target cells differ from BFU-E in velocity and cell cycle characteristics.
- FV target cells and CFU-E show differential sensitivity to AMD and distinct growth kinetics.
- Spleen colonies from FV-infected target cells exhibit increased Ep-independent CFU-E, indicating a primary erythroleukemic lesion.
Conclusions:
- FV-replicating target cells are a distinct erythroid precursor population.
- These cells are partially or not identical to BFU-E and CFU-E.
- FV-infected target cells initiate primary erythroleukemic lesions in vivo.
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