Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Turnover of circulating hematopoietic stem cells.

M J Dorie, M A Maloney, H M Patt

Experimental Hematology

|October 1, 1979

Summary

Hematopoietic stem cell turnover was studied using parabiosis in mice. Donor stem cells rapidly disappeared from circulation, indicating quick blood clearance and marrow equilibration.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Auditory acuity of artillerymen and of rifle coaches.

Archives of otolaryngology·2010

Same author

Hemodynamics in pulmonary irritant poisoning.

The American journal of physiology·2010

Same author

Adrenal response to total body X-radiation.

The American journal of physiology·2010

Same author

Evaluation of certain tests of physical fitness.

The Journal of aviation medicine·2010

Same author

Mitochondrial dysfunction after aerobic exposure to the hypoxic cytotoxin tirapazamine.

Cancer research·2001

Same author

DNA damage measured by the comet assay in head and neck cancer patients treated with tirapazamine.

Neoplasia (New York, N.Y.)·2000

Area of Science:

Hematology
Stem Cell Biology
Mouse Models

Background:

Hematopoietic stem cells (HSCs) are crucial for blood formation.
Understanding HSC dynamics is vital for regenerative medicine.

Purpose of the Study:

To investigate the turnover rate of circulating hematopoietic stem cells.
To determine the kinetics of stem cell disappearance from blood and marrow.

Main Methods:

Short-term parabiosis in CBA/CaJ mice.
Spleen colony assay for stem cell quantification.
Chromosome analysis for donor cell identification.

Main Results:

Circulating pluripotent stem cells exhibited exponential disappearance with a 1.7-hour half-life.
Blood stem cells were in equilibrium with marrow stem cells (9.5-hour half-life).
Splenectomy did not affect stem cell removal rates from blood.

Conclusions:

Circulating hematopoietic stem cells have a rapid turnover rate.
A dynamic equilibrium exists between blood and marrow stem cell populations.
The spleen is not a primary site for stem cell removal from circulation.

Related Experiment Videos