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Microdetermination of naphthionic acid in serum and amniotic fluid
Journal - Association of Official Analytical Chemists
|September 1, 1979
Summary
A new method allows for the simple, rapid, and sensitive measurement of naphthionic acid (NA) in biological fluids. This fluorescence spectrophotometry technique is highly reproducible and suitable for clinical diagnostics.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Clinical Diagnostics
Background:
- Naphthionic acid (NA) is a compound requiring accurate quantification in biological samples.
- Existing methods for NA determination may lack sensitivity, speed, or reproducibility.
Purpose of the Study:
- To develop a simple, rapid, sensitive, and reproducible microdetermination method for naphthionic acid (NA).
- To establish a method for quantifying NA in serum, amniotic fluid, and urine.
Main Methods:
- Protein precipitation using absolute ethanol.
- Measurement of nonderivatized NA via fluorescence spectrophotometry (excitation 328 nm, emission 420 nm).
- Heating samples at 75°C for 30 minutes.
Main Results:
- Detection limit of 1 ng NA in 20 µL serum and 3 ng NA in 50 µL amniotic fluid.
- High reproducibility with standard deviations ranging from 7.59% to 10.1% for various NA concentrations.
- Method allows for approximately 100 determinations daily with results available within 2 hours.
Conclusions:
- The described method provides a reliable and efficient means for naphthionic acid microdetermination.
- The technique is suitable for routine clinical laboratory analysis and diagnostics.
- A modification allows for NA quantitation in urine samples.