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Long-term storage of microorganisms used in antimicrobial effectiveness tests
Journal - Association of Official Analytical Chemists
|September 1, 1979
Summary
Liquid nitrogen storage preserves microbial viability for antimicrobial testing. This method enhances test reliability and maintains high cell viability for up to three years.
Area of Science:
- Microbiology
- Pharmacology
- Biotechnology
Background:
- Accurate antimicrobial effectiveness testing relies on consistent microbial inocula.
- Traditional storage methods can lead to reduced microbial viability and test variability.
Purpose of the Study:
- To describe a method for liquid nitrogen storage of microbial organisms.
- To evaluate the viability and stability of microorganisms stored in liquid nitrogen.
- To assess the impact of liquid nitrogen storage on the reliability of antimicrobial effectiveness tests.
Main Methods:
- Organisms designated by the U.S. Pharmacopeia were subjected to a liquid nitrogen freezing protocol.
- Cell viability was assessed immediately after freezing and at regular intervals.
- Antimicrobial effectiveness tests were conducted using both fresh and cryopreserved inocula.
Main Results:
- Immediate post-freezing viability ranged from 85-95%.
- High viability levels remained constant over a 3-year storage period.
- The use of liquid nitrogen-stored inocula significantly improved test reliability.
Conclusions:
- Liquid nitrogen storage is a superior method for preserving microbial inocula for antimicrobial testing.
- This technique ensures consistent cell viability, enhancing the accuracy and reproducibility of antimicrobial effectiveness assessments.
- The described method offers a reliable and long-term solution for microbial sample preservation.