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Plate assay for detection of Leptospira interrogans serovar pomona hemolysin
Journal of Clinical Microbiology
|October 1, 1979
Abstract:
A plate assay which utilizes the addition of a blood agar overlay for detection of hemolysin by colonies of Leptospira interrogans serovar pomona is described.
Insights
A novel plate assay detects hemolysin produced by Leptospira interrogans serovar pomona colonies. This method uses a blood agar overlay for improved visualization of hemolytic activity.
Area of Science:
- Microbiology
- Bacteriology
Background:
- Leptospira interrogans serovar pomona is a significant bacterial pathogen.
- Hemolysin production is a virulence factor in some Leptospira species.
Purpose of the Study:
- To describe a new plate assay for detecting hemolysin activity.
- To facilitate the identification of Leptospira interrogans serovar pomona.
Main Methods:
- A plate assay was developed.
- The assay incorporates a blood agar overlay.
- Colonies of Leptospira interrogans serovar pomona were tested.
Main Results:
- The blood agar overlay enabled the detection of hemolysin.
- Hemolytic activity was observed from colonies of Leptospira interrogans serovar pomona.
Conclusions:
- The described plate assay is effective for detecting hemolysin.
- This method aids in the characterization of Leptospira interrogans serovar pomona.