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Sexual Crosses with the Mucoromycete Phycomyces blakesleeanus
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A carotenogenic enzyme aggregate in Phycomyces: evidence from quantitive complementation.

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    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1971
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    Phycomyces blakesleeanus mutant strains C2 and C9, when combined, produce varying amounts of carotenoids. This suggests a carotenogenic enzyme assembly line model, with a defective cyclase in strain C9.

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    Area of Science:

    • Biochemistry
    • Mycology
    • Genetics

    Background:

    • Wild-type Phycomyces blakesleeanus synthesizes beta-carotene.
    • Mutant C2 cannot produce carotenoids; mutant C9 produces lycopene.

    Purpose of the Study:

    • To analyze carotenoid production in Phycomyces blakesleeanus heterokaryons.
    • To investigate the mechanism of carotenoid biosynthesis.

    Main Methods:

    • Utilized heterokaryons of Phycomyces blakesleeanus mutant strains C2 and C9.
    • Quantified lycopene, gamma-carotene, and beta-carotene accumulation based on the proportion (p) of C2 nuclei.

    Main Results:

    • Heterokaryons produced lycopene, gamma-carotene, and beta-carotene in relative amounts (1-p), p(1-p), and p^2, respectively.
    • Carotenoid ratios varied predictably with the proportion of C2 and C9 nuclei.

    Conclusions:

    • The observed carotenoid accumulation supports an "assembly line" model for carotenogenic enzyme function.
    • A cyclase enzyme, defective in strain C9, is a key component of this pathway.