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Related Experiment Videos

Localization of two additional hypervariable regions in immunoglobulin heavy chains.

J M Kehoe, J D Capra

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1971
    PubMed
    Summary
    This summary is machine-generated.

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    Researchers identified two new hypervariable regions in immunoglobulin heavy chains (V(H)) and found they may be near the first disulfide bridge. Subgroup distinctions may not span the entire variable region.

    Area of Science:

    • Immunology
    • Protein Chemistry
    • Molecular Biology

    Background:

    • Human IgG myeloma proteins are crucial for understanding immunoglobulin structure and function.
    • The variable heavy (V(H)) chain segment contains hypervariable regions that determine antigen-binding specificity.
    • Previous studies identified one hypervariable region in the V(H) segment.

    Purpose of the Study:

    • To identify and characterize additional hypervariable regions within the V(H) segment of human IgG myeloma proteins.
    • To investigate the spatial arrangement of these hypervariable regions in native immunoglobulin molecules.
    • To determine if V(H) subgroup distinctions are present throughout the entire variable region.

    Main Methods:

    • Isolation and sequencing of cyanogen bromide fragments from heavy chains of human IgG myeloma proteins.

    Related Experiment Videos

  • Automated sequencing methods for fragment analysis.
  • Comparative sequence analysis of human and animal immunoglobulin proteins.

    • Main Results:

    • Two novel hypervariable regions were identified in the V(H) segment (residues 86-91 and 101-109), separated by a conserved region.
    • These hypervariable regions, along with the previously known one (residues 31-37), are suggested to be in close steric proximity due to their relation to the first heavy-chain disulfide bridge.
    • No subgroup-specific residues were found in the terminal portion of the V(H) segment (residue 95 to C(H)1 beginning).

    Conclusions:

    • The V(H) segment contains at least three distinct hypervariable regions.
    • These hypervariable regions are likely positioned close together in the native immunoglobulin structure.
    • Immunoglobulin heavy chain V(H) subgroup distinctions may not be uniformly present across the entire variable region.