Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plastic sectioning for light microscopy of Pyrenomycetes.

S H Mai

    Stain Technology
    |July 1, 1975
    PubMed
    Summary

    A new method using potassium permanganate (KMnO4) fixation and Giemsa staining improves light microscopy of Sordaria fimicola ascocarps. This technique enhances the preservation of delicate fungal structures for detailed morphological studies.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Mechanism for the regulation of post-translational modifications of procollagens synthesized by matrix-free cells from chick embryos.

    The Journal of biological chemistry·1983
    Same author

    Biosynthesis of procollagens and collagens by tissue explants and matrix-free cells from embryonic chick cornea.

    Investigative ophthalmology & visual science·1982
    See all related articles

    Area of Science:

    • Mycology
    • Microscopy Techniques
    • Plant Pathology

    Background:

    • Ascocarps of Sordaria fimicola are crucial for understanding fungal reproduction.
    • Effective light microscopy is essential for detailed morphological studies of fungal structures.
    • Existing methods may not adequately preserve delicate tissues for accurate analysis.

    Purpose of the Study:

    • To develop and optimize a reliable light microscopy preparation method for Sordaria fimicola ascocarps.
    • To improve the preservation of the centrum's delicate sterile tissues.
    • To provide a standardized protocol for future morphological studies of perithecial ascomycetes.

    Main Methods:

    • Ascocarps were embedded in Spurr's medium and sectioned at 1-1.5 µm using an ultramicrotome.
    • Sections were stained with Giemsa solution at 60°C for 10-30 minutes.
    • Optimal fixation involved 1% potassium permanganate (KMnO4) for 2.5-3 hours prior to Giemsa staining.

    Main Results:

    • The optimized method successfully preserved the delicate sterile tissues of the ascocarp centrum.
    • Giemsa staining following KMnO4 fixation provided clear visualization of fungal morphology.
    • The protocol yielded high-quality sections suitable for light microscopy.

    Conclusions:

    • A refined protocol combining KMnO4 fixation and Giemsa staining is effective for light microscopy of Sordaria fimicola.
    • This method enhances the morphological study of perithecial ascomycetes.
    • The proposed technique is recommended for future research on fungal morphology.

    Related Experiment Videos