Use of affinity chromatography for purification of tyrosinase

The possibility of using affinity chromatography for the purification of tyrosinase was explored. When purified mushroom tyrosinase was employed, almost all tyrosinase became bound to Sepharose containing 3-iodotyrosine, an inhibitor of tyrosinase. When crude extracts from B16 melanoma were employed, partial purification of tyrosinase and separation of two or possibly three forms of tyrosinase could be obtained. The results indicate that affinity chromatography could possibly be employed for the purification of tyrosinase during the final stages of the purification procedures.